Scaffold Application Notes

Cell culture scaffolds based on concentric shape constructs, such as circles, provide a consistent variability of pore size (heterogenous pore size and distribution) emanating from the centre to the periphery of said scaffold. The effect is to provide clear regions of the scaffold where cells will have more favourable nutrient and oxygen exchange (periphery) and areas not so (centre). Such a construct better represents physiological conditions in the body providing tangible experimental advantages for research in fields studying cell microenvironments.

Presented below are protocols to enable efficient deployment of our scaffolds for biological research

Note: protocols are applicable to scaffolds placed in 24 Well Plates
media volumes for example should always be adjusted when utilzing alternative well plates to ensure the scaffolds are fully immersed.

SCAFFOLD PROSPECTUS

In-Vitro Cell Culture scaffold

COLLAGEN PROTOCOL

Collagen Coating Protocol

FIBRONECTIN PROTOCOL

Fibronectin Coating Protocol

ORGANOID and SPHEROID PROTOCOL

Organoid and Spheroid Protocol