Design and manufacture of cell culture scaffolds based on concentric shape constructs, such as circles, providing a consistent variability of pore size (heterogenous pore size and distribution) emanating from the centre to the periphery of said scaffold. The effect is to provide clear regions of the scaffold where cells will have more favourable nutrient and oxygen exchange (periphery) and areas not so (centre). Such a construct better represents physiological conditions in the body providing tangible experimental advantages for research in fields studying cell microenvironments.

The total surface area and pore sizes exhibited by the scaffolds depend on the overall structure of the scaffold. This includes the number and size of the supports/struts within the support layers, the pattern in which the supports/struts are placed,the geometry of the concentric shapes layers, and the order and combinations of layers employed.

Shape constructs within concentric shape layers can be spaced apart in a controlled fashion, as selected by the designer, in order to define the open channel dimensions between shape constructs.

The space/gap between concentric shape layers as realised by the support layers can range from small (50-100 microns) to large (100-400 microns) or a mixture of the two.

The pore sizes within the complete scaffold vary, ranging anywhere from 10 to 1000 microns. The pores of the scaffold are all interconnected, promising an internal space for cells to infiltrate and adhere, either to one another, or the internal/external surface of the scaffold.

Using our bespoke Bio Scaffold modelling software our designers can produce scaffolds to your required specification.

Alternatively our library 3D PETG Scaffolds having consistent structure, with heterogenous porosity are available to purchase through our distributors Appleton Woods (UK and Ireland), Lucurna-Chem AG (Switzerland) and ISOGEN Life Science (Benelux and Apain)